Fluorescent indicator displacement assays to identify and characterize small molecule interactions with RNA

Application of Small Molecule Receptors to the Analysis of Post- Translational Modifications Using Fluorescent Indicator Displacement Assays

Fluorescent peptide indicator displacement assay for monitoring interactions between RNA and RNA binding proteins.

This paper describes a sensitive, non-destructive displacement assay, using a fluorescent peptide indicator, for real-time monitoring of the interactions between RNA and RNA binding proteins (RBPs). The developed fluorescent peptide indicators, each containing a mid-sequence fluorophore unit, allowed sensing of target RNA and RNA-RBP interactions through changes in fluorescence intensity. We an...

Small-molecule fluorescent probes for specific RNA targets.

A method was developed that uses small molecules as fluorescent probes to detect specific mRNAs. In this approach, the fluorescence of fluorophore-quencher conjugates is restored by the binding of an mRNA aptamer tag to the quencher segment of the molecules. The method allows real-time detection of mRNA transcripts in vitro.

Transcript profiling and RNA interference as tools to identify small molecule mechanisms and therapeutic potential.

The identification of the mechanism of action and therapeutic potential of bioactive small molecules remains a considerable challenge in the field of drug discovery and chemical biology. Apart from traditional target identification techniques, new tools have emerged that can significantly aid mechanism elucidation efforts. The development of pattern matching algorithms that compare transcriptio...

Indicator displacement assays that detect bilayer membranes enriched in phosphatidylserine

Three indicator displacement assays are described for the detection of phosphatidylserine in a bilayer membrane. A series of Zn-dipicolylamine coordination compounds are used to bind selectively to the phosphatidylserine and act as a colorimetric chemosensing ensemble when combined with the UV-Vis indictor pyrocatechol violet. A similar displacement assay uses a coumarin methylsulfonate derivat...